This book provides a complete overview of the fundamentals of bioprinting and also covers recent advancements and future applications. Most treatment on the topic of bioprinting only focuses on a particular area, such as printing methodologies or printable inks, within the topic. However, bioprinting is very broad and interdisciplinary area that integrate many rapidly growing technologies such as data science, machine learning, and materials informatics to support industry 4.0. The first section of the book provides essential background information in the field. of the established field such as cellular requirements and preparation for bioprinting, materials, printability and shape fidelity, and toxicity aspects and ethical issues of bioprinting. The second section moves on to relevant innovations associated with the field including controlling factors of bioprinting (patterning and surface topography), in situ bioprinting, impact of machine learning. The book even touches on advanced bioprinting for future advances like organ on chip system, Five-dimensional (5D) printing in the medical field, dynamic tuning of ink rheological properties, benefits of multi-material extrusion approach, space bioprinting-gravity-free effect, and more.