PART I TRENDS IN VACCINE DEVELOPMENT 1. Artificial Intelligence for Vaccine Design Peter McCaffrey 2. Progress in the Development of Structure-Based Vaccines Sunil Thomas and Ann Abraham 3. Polymer-Peptide Conjugate Vaccine for Oral Immunization Mohammad Omer Faruck, Mariusz Skwarczynski, and Istvan Toth 4. An update on "Reverse Vaccinology" The Pathway from Genomes and Epitope Predictions to Tailored, Recombinant Vaccines Marcin Michalik, Bardya Djahanshiri, Jack C. Leo, and Dirk Linke PART II VACCINE VECTORS AND PRODUCTION SYSTEM 5. Phage T7 as a Potential Platform for Vaccine Development Chuan Loo Wong, Chean Yeah Yong and Khai Wooi Lee 6. Plant-based Systems for Vaccine Production Mattia Santoni, Elisa Gecchele, Roberta Zampieri, and Linda Avesani 7. Production of a Hepatitis E Vaccine Candidate Using the Pichia pastoris Expression System Jyoti Gupta, Amit Kumar, and Milan Surjit PART III VACCINE ADJUVANTS 8. Developments in Vaccine Adjuvants Farrhana Ziana Firdaus, Mariusz Skwarczynski, and Istvan Toth 9. Adjuvants: Engineering Protective Immune Responses in Human and Veterinary Vaccines Bassel Akache, Felicity C. Stark, Gerard Agbayani, Tyler M. Renner and Michael J. McCluskie 10. Cationic Nanostructures as Adjuvants for Vaccines Ana Maria Carmona-Ribeiro, Beatriz Ideriha Mathiazzi, and Yunys Pérez-Betancourt 11. Emulsion Adjuvants for Use in Veterinary Vaccines Rachel Madera, Yulia Burakova, and Jishu Shi 12. Generation of a Liposomal Vaccine Adjuvant Based on Sulfated S-lactosylarchaeol (SLA) Glycolipids Bassel Akache, Yimei Jia, Vandana Chandan, Lise Deschatelets and Michael J. McCluskie 13. Glucan particles: choosing the appropriate size to use as a vaccine adjuvant Mariana Colaço, João Panão Costa, and Olga Borges PART IV VACCINE DELIVERY SYSTEMS 14. Use of Optical In Vivo Imaging to Monitor and Optimize Delivery of Novel Plasmid-Launched Live-Attenuated Vaccines Sapna Sharma and Kai Dallmeier 15. Liposomes for the Delivery of Lipopeptide Vaccines Jieru Yang, Armira Azuar, Istvan Toth, and Mariusz Skwarczynski 16. Current Prospects in Peptide-Based Subunit Nanovaccines Prashamsa Koirala, Sahra Bashiri, Istvan Toth, and Mariusz Skwarczynski 17. Design and Synthesis of Protein-based Nanocapsule Vaccines Ivana Skakic, Jasmine. E. Francis, and Peter. M. Smooker 18. Design and Preparation of Solid Lipid Nanoparticle (SLN) Mediated DNA Vaccines Jasmine. E. Francis, Ivana Skakic, and Peter. M. Smooker 19. Nano-Particulate Platforms for Vaccine Delivery to Enhance Antigen-Specific CD8+ T Cell Response Jhanvi Sharma, Carcia S. Carson, Trevor Douglas, John T. Wilson, and Sebastian Joyce 20. PilVax: A Novel Platform for the Development of Mucosal Vaccines Catherin