Name: Experiments in Bacterial Genetics: A Laboratory Manual (en Inglés)
Price: 440365.00 COP
Availability: InStock
Author: Bossi/Camilli/Grundl
ISBN: 9781621824497

portada Experiments in Bacterial Genetics: A Laboratory Manual (en Inglés)

Formato

Libro Físico

Autor

Bossi/Camilli/Grundl

Editorial

Cold Spring Harbor Laboratory Press

Idioma

Inglés

N° páginas

352

Encuadernación

Tapa Blanda

Dimensiones

27.7 x 21.7 x 2.0 cm

Peso

0.89 kg.

ISBN13

9781621824497

Categorías

Equipamiento, experimentos y técnicas científicas
Genética (no médica)

Experiments in Bacterial Genetics: A Laboratory Manual (en Inglés)

Bossi/Camilli/Grundl (Autor) · Cold Spring Harbor Laboratory Press · Tapa Blanda

Libro Físico

$ 440.365

$ 880.731

Ahorras: $ 440.365

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Origen: Estados Unidos (Costos de importación incluídos en el precio)

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Reseña del libro "Experiments in Bacterial Genetics: A Laboratory Manual (en Inglés)"

Bacteria are powerful systems for genetic research. They are fast growing and easy to propagate and maintain, and many tools have been developed for their genomic analyses and genetic manipulation. The study of bacteria and their viruses allowed deciphering the genetic code and has shaped our understanding of fundamental biological processes such as DNA replication, transcription, and translation. This manual provides a collection of experimental procedures for genetic research in bacteria. It describes basic methods for the isolation and propagation of bacteria and phages, purification of genomic DNA and plasmids, and introduction of DNA into bacteria. Diverse strategies for generating and confirming mutations and for creating gene deletions and gene fusions are described, using allelic exchange, random transposition, recombineering, and CRISPR-Cas technologies. Protocols for bacterial genome sequencing and resequencing, as well as for high-throughput mutant screening via transposon sequencing, are provided. Although specific bacterial species such as Escherichia coli, Salmonella enterica, Vibrio cholerae, and Staphylococcus aureus are used for some of the protocols, the methodologies herein described can readily be adapted for use with other bacteria. This manual thus represents a valuable resource for those seeking to perform genetic experiments in bacteria.