Base Editors: Methods and Protocols (Methods in Molecular Biology, 2606) (en Inglés)
Reseña del libro "Base Editors: Methods and Protocols (Methods in Molecular Biology, 2606) (en Inglés)"
Part I: Overview of BEs, Diverse Variants of BEs, and Computational Tools for Bes 1. Introduction and Perspectives of DNA Base Editors Beomjong Song and Sangsu Bae 2. Web-Based Computational Tools for Base Editors Gue-Ho Hwang and Sangsu Bae 3. Prediction of Base Editing Efficiencies and Outcomes Using DeepABE and DeepCBE Jinman Park and Hui Kwon Kim 4. Profiling Genome-Wide Specificity of dCpf1 Cytidine Base Editors Using Digenome-Seq Daesik Kim Part II: Application of BEs for Disease Modeling 5. Base Editing of Human Hematopoietic Stem Cells Jing Zeng, Gabriele Casirati, My Anh Nguyen, Pietro Genovese, and Daniel E. Bauer 6. A/C Simultaneous Conversion Using the Dual Base Editor in Human Cells Xiaohui Zhang, Yuting Guan, and Dali Li 7. Functional Analysis of Variants in BRCA1 Using CRISPR Base-Editors Ji-Eun See and Yongsub Kim 8. Use of the Representative Base Editing Tool Target-AID to Introduce Pathogenic Mutations into Mice Hiroki Sasaguri 9. Targeted Mutagenesis in Mice Using a Base Editor Tae Yeong Jeong, Soo-Yeon Lim, Je Kyung Seong, and Kyoungmi Kim Part III: Therapeutic Base Editing Strategies 10. Heterologous Expression and Purification of a CRISPR-Cas9-Based Adenine Base Editor Seu-Na Lee, Hong-Su Jang, and Jae-Sung Woo 11. Delivering Base Editors In Vivo by Adeno-Associated Virus Vectors Colin K.W. Lim, Angelo J. Miskalis, Pablo Perez-Pinera, and Thomas Gaj 12. The Delivery of ABE mRNA to the Adult Murine Liver by Lipid Nanoparticles (LNPs) Dong Yang and Chun-Qing Song 13. Ex Vivo Base Editing Therapy with Chemically-Derived Hepatic Progenitors Yohan Kim, Jaemin Jeong, and Dongho Choi 14. Application of Base Editor-Mediated Genome Editing in Mouse Retina Jun Wu, Chang Sik Cho, Dong Hyun Jo, and Jeong Hun Kim Part IV: Use of BEs in Plants and Bacteria 15. High-Throughput Base-Editing-Mediated Artificial Evolution Streamlines Trait Gene Identification in Rice Fang Yan, Man Yu, Meixia Wang, and Huanbin Zhou 16. Heritable Virus-Induced Genome Editing (VIGE) in Nicotiana attenuate Hyeonjin Kim, Youngbin Oh, Eunae Park, Moonyoung Kang, Yuri Choi, and Sang-Gyu Kim 17. Cytosine Base Editing in Bacteria
